Hygeia journal for drugs and medicines


Phytochemical, HPTLC finger printing and antibacterial activity of Acacia nilotica (L.) Delile
Hygeia.J.D.Med.Vol.2 (2), 2010, 38-42.  

Type of article: research article

Authors: R.Venkataswamy1,  A.Doss2, H. Mohamed Mubarack2 , M.Sukumar1  .


1. Department of Pharmacognosy, Sri Ramakrishna College of Pharmacy , Coimbatore , Tamil Nadu , India .

2. Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore, Tamil Nadu, India .

Article history: 

Received:  5 April 2010, 
Revised:  16 May 2010,
Accepted: 22 June 2010,
Available online: 31 July 2010. 


The leaves of Acacia nilotica (L.) Delile was extracted with methanol and aqueous medium and studied for in vitro antimicrobial property. The methanol extract was found to be most active against all the bacterial species tested except S.aureus. Active methanol extract was further studied for HPTLC fingerprint and by phytochemical analysis. HPTLC analysis confirmed segregation of four individual compounds with individual Rf values and peak area percentage. The results of phytochemical screening of extract revealed the presence of Tannins, Carbohydrates and Glycosides. This analysis revealed the high antibacterial activity in the methanol extract of Acacia nilotica (L.) Delile

Key words: Acacia nilotica (L.) Delile , HPTLC finger printing, methanol, MIC, microorganisms

Article outline:

1.  Introduction
2.  Materials and methods
     HPTLC finger printing.
     Phytochemical Analysis
     Antibacterial Screening
     Minimum Inhibitory Concentration
3.  Results and discussion
4.  References

Graphical outline:

HPTLC finger print of the methanolic extract of
Acacia nilotica (L.) Delile

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© 2010 Hygeia journal for drugs and medicines.All rights reserved. 0975 6221 , 2229 3590


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