Hygeia journal for drugs and medicines


Visible Spectrophotometric method for the estimation of Cefepime
Hygeia.J.D.Med.Vol.2 (2), 2010, 32-37.  

Type of article: research article

Authors: Minu Sujith1, Sujith Abraham1*and Madhu.C.Divakar2


1. Nirmala College of Pharmacy, Muvattupuzha, Ernakulam , Kerala , India - 686661
2. Crescent College of Pharmaceutical Sciences ,Payangadi.P.O , Kannur, Kerala , India  670358 .
Article history: 

Received: 10 January2010,,
Revised: 25April2010,
Accepted:18 june2010 ,
Available online 31 July 2010.


A simple, accurate and reproducible visible spectrophotometric method was developed for the estimation of cefepime in pure and vial formulation. Cefepime with 1-Chloro-2,4-dinitrobenzene in presence of dimethyl sulfoxide at 45oC have formed a yellow colored complex having maximum absorbance at 420nm.The calibration graph was linear in the range 20µg/ml to50µg/ml.

KeywordsVisible spectrophotometry, Cefepime, 1-Chloro-2, 4-dinitrobenzene, Dinitrobenzene

Article outline:

1.  Introduction
2.  Experimental
  2.1. Instruments used
  2.2. Chemicals and reagents
  2.3. Preparation of standard drug solution
  2.4. Analysis of pharmaceutical formulation
  2.5. Recovery studies
3. Results and discussion
4. Conclusion

Graphical outline:
Beer's -Lambert's plot of cefepime at 420 nm

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