Abstract
SYNTHESIS, ANTIMICROBIAL EVALUATION AND FORCED DEGRADATION STUDIES OF MUTUAL AMIDE PRODRUG OF OFLOXACIN AND ISONIAZID
Objective: The aim of the present work was to synthesize highly potent antitubercular agent and evaluate the antimicrobial activity of mutual amide prodrug of Ofloxacin with Isoniazid.
Method: The mutual prodrug was synthesized by single step process by the condensation of Ofloxacin with Isoniazid in the presence of POCl3 with continuous stirring for 3 hrs. The structure was characterized by IR spectroscopy and thin layer chromatography. Forced degradation studies were performed under various stress conditions like acid, alkali, oxidation, photolytic and thermal. The mutual prodrug was tested for antibacterial and antifungal activity by using cup plate method and serial dilution methods against the standard drugs Metronidazole and Amikacin respectively.
Outcome: The developed method was simple and effective method for synthesis and the technique can be further used for bulk preparation and for the quality control of the prodrug. The results from the study showed that the drug was stable in alkaline condition.