Hygeia journal for drugs and medicines


Development and validation of an RP- UFLC method for the estimation of Dexchlorpheniramine maleate.

Hygeia.J.D.Med 2018; 9(2): 26-33
DOI: 10.15254/H.J.D.Med.9.2018.171

Shanmugam R1, Murali P 2*, Lalitha Priyanka D3, Madhuri K1 , Ashok Kumar CK1 .

1. Department of Pharmaceutical Analysis, Sree Vidyanikethan College of Pharmacy, Tirupati-517102, India.
2.  Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109

3. Department of Pharmacognosy, Seven Hill College of Pharmacy, Tirupati, India

Plan: Dexchlorpheniramine maleate is a Chlorpheniramine dextrorotatory isomer, which is twice active than Chlorpheniramine. It’s widely used in the coughs, cold and allergic conditions including angioedema, urticaria, conjunctivitis, rhinitis, and pruritic skin disorders. The main objective of the present research work deals with the development of simple, rapid and sensitive RP- UFLC analytical method for estimation of Dexchlorpheniramine maleate in bulk and finished formulation.

Methodology:  simple, rapid and sensitive RP- UFLC analytical method was developed. Optimized chromatographic separation condition was done by using an isocratic mode with the mobile phase of 25 mM of potassium dihydrogen ortho phosphate (pH 4) and acetonitrile at the ratio of 80:20v/v with a flow rate of 1ml/min. Stationary phase used was C18 column (250 x 4.6 mm). Dexchlorpheniramine maleate detection was carried at 260 nm. The developed method was evaluated for the validation parameters as per the ICH guidelines.

Outcome: The developed optimized chromatographic condition was achieved and results showed good peak resolution. The developed method for the Dexchlorpheniramine maleate can be used for the qualitative and quantitative estimation of finished and bulk formulations.

Key words: Dexchlorpheniramine maleate, Method development, Validation, UFLC


Murali P. Master of Research,
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109


Article History

Received: 9 September 2017,   
Revised: 25 September 2017

Accepted: 11 October 2017, 
Available online: 4 January 2018

Article citation 

Shanmugam R, Murali P*, Lalitha Priyanka D, Madhuri K, Ashok Kumar CK. Development and validation of an RP- UFLC method for the estimation of dexchlorpheniramine maleate. Hygeia.J.D.Med 2018; 9(2)26-33:.Available from http://www.hygeiajournal.com 10.15254/H.J.D.Med.9.2018.171


           Dexchlorphenirarnine maleate

HPLC chromatogram of 
Dexchlorphenirarnine maleate


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