Abstract
Development and Validation of an Antidiabetic Polyherbal Formulation Containing Curcumin Using RP-UFLC Method.
Hygeia.J.D.Med.6(2) 2014; 36-44.
Rashmi N G, Gurupadayya B M, Sirisha Tadiboyina, Jinesh B Nagavi,Chetan G Shinde
Departmentof Pharma. Chemistry, JSS College of Pharmacy, JSS University, Mysore,Karnataka, India.
Plan:Theprinciple destination of our work was to develop a simple, rapid and sensitivereverse phase ultra-fast liquid chromatographic (RP-UFLC) method for estimationof curcumin in anti-diabetic poly herbal formulation (Mehagni).
Methodology: Chromatography wascarried on a reverse phase C18 column (250 x 4.6 mm) with themixture of methanol and 2% acetic acid as a mobile phase at the proportion of70:30 v/v with the flow rate of l.2 ml/min. The absorbance measured at 420 nmby PDA detector.
Outcome: Optimized chromatographic conditions were achieved andresults showed good peak resolution. The retention time was found at 5.02 min.The method was validated as indicated by International Conference onHarmonization guidelines. The parameters, such as specificity, sensitivity,linearity, precision, accuracy, ruggedness, robustness and system suitabilitywere performed. The framework was linear with a correlation co-efficient of0.9945. %RSD of system and method precision were found to be 1.14 and 1.13. TheLOD & LOQ for curcumin was found to be 0.2 µg/ml and 0.65 µg/ml.
Keywords: Curcumin, Mehagni, RP-UFLC, Anti- Diabetic Herbal Formulation.
Received: 5 July 2014, Revised: 20 July 2014, Accepted: 30 July2014, Available online: 10 October 2014
RashmiN G, Gurupadayya B M, Sirisha Tadiboyina, Jinesh B Nagavi, Chetan G Shinde. Development and validation of anantidiabetic polyherbal formulation containing curcumin using RP-UFLC method.Hygeia.J.D.Med 2014; 6(2):36-44. Available from http://www.hygeiajournal.com / Article ID-Hygeia.J.D.Med/134/14.doi:10.15254/H.J.D.Med.6.2014.134.
Correspondence:
Rashmi N G, Departmentof Pharma. Chemistry, JSS College of Pharmacy, JSS University, Mysore,Karnataka, India.
Email: ng.rashmi85@gmail.com