Abstract

Phytochemical, Cytotoxic, anti-Herpes Simplex Virus type 1 (HSV-1) and anti bacterial studies of Terminalia laxiflora Engl. and Diels

Hygeia.J.D.Med.5 (2) 76-86

KhaledRashed1^, Lucy Ono2

1. National Research Centre, Pharmacognosy Department, Dokki, Giza, Egypt.
2.Yasuyoshi Hayashi Microbiology Laboratory, Department of Basic Pathology, UFPR, Curitiba, PR, Brazil.

Article ID- Hygeia.J.D.Med/112/13.

Articlehistory: Received: 10 August 2013,   revised: 27 August 2013,accepted: 2 Sept. 2013, Available online: 3 October 2013 

ABSTRACT

Plan: The aim of the present research was focused on cytotoxic, antiviral and antibacterial properties and the main phytoconstituents of Terminalia laxiflora leaf methanolic extract.

Methodology: The methanolic 80%extract of Terminalia laxiflora leaves was tested for cytotoxicityevaluation in Vero cells in-vitro, anti-HSV-1 activity and antibacterialactivity determinations against some bacteria strains and alsophytoconstituents were detected.

Outcome: The results showed that T. laxiflora leaves methanol 80% extract showed CC₅₀= 1851 μg/mLand also inhibited completely the development of the HSV-1 induced cytopathic effect at concentration of 238 μg/mL while the extract had no effect on bacterial strains. Phytochemical analysis of theextract revealed the presence of carbohydrates, tannins, flavonoids, alkaloids,triterpenes and chromatographic separation of the extract of T.laxiflora leaves resulted in the isolation and identification ofβ-sitosterol, m-gallate, gallic acid, ellagic acid and five flavonoids,quercetin, vitexin, iso vitexin, quercetin 3-O-α-rhamnoside and rutin. Thisresearch may be useful in the development of new and effective antiviral agents.

Keywords: Terminalia laxiflora, leaves, cytotoxicity, anti-HSV-1, antibacterial, phytoconstituents,methanolic extract

Khaled Rashed,Lucy Ono, Phytochemical, Cytotoxic, anti-Herpes Simplex Virus type 1 and antibacterial studies of Terminalialaxiflora Engl. and Diels. Hygeia.J.D.Med. 2013; 5(2): page.76-86. Available from   http://www.hygeiajournal.com/ Article ID- Hygeia.J.D.Med/112/13.