Abstract

Estimation of Lomefloxacin hydrochloride in Bulk and Tablet dosage form by HPTLC method

Hygeia.J.D.Med. 2013; 5(1):141-147

Rajasree RS1*, K. Radha1, Shyni.Bernard1, K.N.Girija1, A.K.C Nair 2.

Article history: Received: 11 January 2013,   revised: 21 January 2013, accepted: 5February2013, Available online: 3 April 2013

Abstract

Plan: An analytical method for the estimation of Lomefloxacin in bulk drug and pharmaceutical dosage forms by HPTLC is described.

Prologue: The method is simple, fast and accurate and can be used for routine analysis of commercial Lomefloxacin tablets.

Methodology: The developed method used precoated silica gel 60F ₂₅₄ as stationary phase. The mobile phase used was a mixture of 2-Propanol: water: ammonia (86:8:6 %v/v/v).The RF value was 0.14.The detection of spots was carried out at 288nm. The calibration curve was found to be linear between 10 and 70 ng/spot with a correlation coefficient of 0.9969.  The limit of detection (LOD) and limit of quantification (LOQ) were found to be 5 ng/spot and 10 ng/spot respectively.

Outcome: The proposed method has been applied successfully for the determination of Lomefloxacin in pharmaceutical dosage forms. No significant interference was observed from excipients, coloring and flavoring agents commonly used in the formulation. The mean recovery of drug from tablets was in the range of 100.8% to 102.7 %.

Key Words:HPTLC, Lomefloxacin , Mobile phase, Stationary phase.

Rajasree RS, K. Radha,Shyni.Bernard, K.N.Girija, A.K.C Nair. Estimation of Lomefloxacinhydrochloride in Bulk and Tablet dosage form by HPTLC method. Hygeia.J.D.Med. 2013; 5(1):141-147.Available at http://www.hygeiajournal.com/ ArticleID- Hygeia.J.D.Med/99/13