Hygeia journal for drugs and medicines


Simultaneous Estimation of Atorvastatin Calcium and Amlodipine besylate by UV Spectrophotometric method using hydrotropic solubilization

Hygeia.J.D.Med2013; 5(1):105-112

Shyni Bernard 1*, Molly Mathew 2, K.L.Senthilkumar3, K.N.Girija1

  1. College of Pharmaceutical Sciences, Govt.Medical College, Kottayam, Kerala-686 008.

  2. Malik Deenar College of Pharmacy, Kasaragod, Kerala-671 321

  3. Padmavathy College of Pharmacy, Dharmapuri, Tamilnadu-635 205.

Article  history: Received: 1 January 2013,  revised: 11 January 2013, accepted: 13 February 2013, Available online:3 April 2013


Plan: An analytical method for the simultaneous estimation of Atorvastatin Calcium (ATV) and Amlodipine Besylate (AML) by UV spectrophotometry using hydrotropic solubilization is described.

Prologue: The method is simple, fast and accurate and can be used for the routine analysis of Atorvastatin and Amlodipine in combination tablets.

Methodology: The developed method uses the absorption ratio or Qvalue  which is based on the  measurement of absorptivity at 293 nm (iso-absorptive point, both the drugs were found to have same absorbance at this wave length) and 247 nm (absorption maximum of one of the drugs, Atorvastatin). Both the drugs are insoluble in water and require  corrosive organic solvents for solubilization. Therefore an attempt was made to preclude the use of corrosive solvents by the use of   2M Urea by hydrotropic solubilization method. The calibration curves for both drugs were found to be linear in a concentration range of 10-60µg/mL .

Outcome: The proposed method has been applied successfully for the simultaneous determination of Atorvastatin and Amlodipinein pharmaceutical dosage forms. No significant interference was observed from the tablet excipients and 2M Urea used for solubilization. The mean recovery of the drugs from the combination tablets was 100.65%.for Atorvastatin and 101.42% for Amlodipine respectively.

Key Words: Amlodipine, Atorvastatin, Spectrophotometry, Tablets.



Shyni Bernard, Molly Mathew, K.L.Senthilkumar, K.N.Girija. Simultaneous Estimation of Atorvastatin Calcium and Amlodipine besylate by UV Spectrophotometric method using hydrotropic solubilization. . Hygeia.J.D.Med2013; 5(1):105-112. Available at  http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/94/13




For Correspondence: shinjopin@yahoo.co.in
 Hygeia.J.D.Med. Vol.5 (1), April 2013
© 2013, Hygeia journal for drugs and medicines
All rights reserved.  2229 3590, 0975
Researcher ID: B-8471-2013

Overlain absorption spectrum  of Atorvastatin and
Amlodipine in 2M Urea solution


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