Hygeia journal for drugs and medicines


Protective effect of DHC-1, a Polyherbal formulation, against CCl4 induced Liver damage.


Bafna PA1 and Balaraman R2*

1. Rayat Institute of Pharmacy, Rayat and  Bahra Campus, Railmajra, Dist. Nawanshahar,Punjab – 144 533 INDIA.
2.Department of Pharmacy, Sumandeep University, A/P Pipariya, (Waghodia),Vadodara-391760, Gujarat, INDIA.


Article history: Received: 15 November 2012,   revised: 21 December 2012, accepted: 17 January 2013, Available online: 3 April 2013


Plan: DHC-1, a standardized poly herbal formulation, was investigated for its hepatoprotective and antioxidant effects against CCl4-induced liver damage in rats.

Methodology: DHC-1at various doses (125, 250, 500 and 1000 mg/kg, p.o.) was studied for its effect on the levels of SGPT, SGOT, alkaline phosphatase and total bilirubin.The drug was also evaluated for its effect on markers of tissue oxidative stress, namely extent of lipid peroxidation (MDA), levels of enzymatic antioxidants (SOD and catalase), non-enzymatic antioxidant (GSH) and membrane bound enzymes (Ca2+ATPase, Mg2+ATPase and Na+K+ATPase).

Outcome: Reduction in the levels of serum markers of liver damage; and decrease in tissue MDA levels and increase in SOD, catalase, GSH and membrane bound enzymes indicated the hepatoprotective and antioxidant property of DHC-1. Thus it is evident that DHC-1, at least partly by virtue of its antioxidant activity, elicited protective effects on the liver against the oxidative damage induced by CCl4.

Keywords: Polyherbal formulation;Hepatoprotective; CCl4; lipid peroxidation, superoxide dismutase,catalase, reduced glutathione.


Bafna PA, Balaraman R. Protective effect of DHC-1, a Poly herbal formulation, against CCL4 induced Liver damage. Hygeia.J.D.Med2013;5(1):10-18.Available from   http://www.hygeiajournal.com /Article ID- Hygeia.J.D.Med/83/13



For Correspondence:rbalaraman2000 @yahoo.com 

Hygeia.J.D.Med.Vol.5 (1), April 2013
©2013, Hygeia Journal for drugs and medicines, 
All rights reserved.  22293590, 09756221

Researcher id: K-6431-2012


  • watch Video
    Collagen Mimetic Peptides
  • watch Video
    Mechanisms and secrets of Alzheimer's disease...
  • watch Video
    Immunotherapy to fight cancer
  • watch Video
    Food Safety Emergency Prevention
  • watch Video
    Tiny worm-like robot could deliver medicine inside body
  • watch Video
    This smart pill could crawl through your body to deliver its medicine
  • watch Video
    Introduction To Nanorobotics: A conversation