Hygeia journal for drugs and medicines


An Activity guided Isolation and Evaluation of various Solvent Extracts of the leaves of Anjan Grass

Premlata Singariya1*, Padma Kumar1and Krishan Kumar Mourya2

1. Laboratory ofTissue Culture and Secondary Metabolites, Department of   Botany, University of Rajasthan,Jaipur- 302004,India.

2.Govt. Veterinary Hospital, Pahari (Bharatpur) Rajasthan, India.

Article history:Received: 22 July 2012,   revised: 1 August 2012, accepted: 19 September 2012, Available online:  October 2012


Plan: The present study was undertaken to identifythe possible bioactive components andantimicrobial activity in variouspolar solvents from the leaves of Anjan grass (Cenchrus ciliaris) (Poaceae).

Materials and methods: New compounds were identified by GC-MS analysis. Antimicrobial activitywas evaluated against Proteus mirabilis,Klebsiella pneumoniae, Agrobacterium tumefaciens and Aspergillus niger using diskdiffusion method followed by MIC by broth dilution method.

Outcome:Various aromatic, steroids, fatty acids andesters from C. ciliariswere identified. The prevailing compounds in the ethyl acetate extract of C. ciliaris were 4,22-stigmastadiene-3-one (2.41%),cyclopentacycloheptene(azulene) (1.81%), ergost-5-en-3-ol (3β, 24R)- (campesterol)(1.63%) and myristic acid, isopropyl ester(0.17%). The highest antimicrobial activity was exhibited by the glacialacetic acid extract against P. mirabilis. Thepresence of various bioactive compounds justifies the use of this plant forvarious ailments by traditional practitioners. However, isolation of individualphotochemical constituents and subjecting it to biological activity willdefinitely give fruitful results.

Keywords: Antibacterial activity, Azulene, Campesterol, Cenchrus grass and Myristic acid,isopropyl ester.



Hygeia.J.D.Med.Vol.4 (2), Oct. 2012 – March 2013

©2012, Hygeia journal for drugs and medicines, all rights reserved.  2229 3590, 0975 6221 


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