Abstract

Cytotoxicity studies of Tamus communis root and berries extracts on human HEp-2 and AMN-3 carcinoma cells

 Ekbal Al-Khateeb, Ara Adkar Ohan^*,Hakeem Al-Ani        

Article history: Received: 24 May 2012,   revised:15 June 2012, accepted:30 June 2012, Available online:10 October 2012

Page:1-5

Abstract

Plan

 In the present study, the steroidal fraction was isolated from the ethanolic extract of T.communis root and berries (TCRE &TCBE, % yield: 0.035 and 0.032 respectively) and screened for their cytotoxicity against human epidermoid laryngocarcinoma (HEp-2) and Ahmed-Mohammad-Nahi-2003 mammary adenocarcinoma (AMN-3) cell lines.  

Methodology

Tamus communis (F: Dioscoreaceae) is a plant, growing wildly in the northern part of Iraq, mainly contains the steroidal sapogenin, diosgenin, in its roots and berries. In the present study, the steroidal fraction was isolated from the ethanolic extract of T.communis root and berries (TCRE &TCBE, % yield: 0.035 and 0.032 respectively) and screened for their cytotoxicity against human epidermoid laryngocarcinoma (HEp-2) and Ahmed-Mohammad-Nahi-2003 mammary adenocarcinoma (AMN-3) cell lines. The presence of diosgenin was confirmed in the extract by HPLC methods with standard diosgenin sample.

Outcome

The plant extracts, TCRE and TCBE showed better cytotoxic effect (IC₅₀ 342.56 μg/ml and 386.55µg/ml) on HEp-2 cell line than pure diosgenin (IC₅₀ 409.29 μg/ml), while diosgenin was more toxic to AMN-3 cell lines (IC₅₀ 360.04μg/ml) compared to the plant extracts (TCRE: IC50 400.28 and TCBE: 465.64μg/ml respectively).

Key words: AMN-3 cell lines, HEp-2 cell lines, cytotoxicity, TCRE, TCBE, diosgenin

How to cite this article: Ekbal Al-Khateeb, Ara Adkar Ohan,Hakeem Al-Ani.Cytotoxicity studies of Tamus communis root and berries extracts on human HEp-2 and AMN-3 carcinoma cells. Hygeia.J.D.Med 2012;4(2):1-5.

How to cite this URL:. Ekbal Al-Khateeb, Ara Adkar Ohan, Hakeem Al-Ani.Cytotoxicity studies of Tamus communis root and berries extracts on human HEp-2 and AMN-3 carcinoma cells.Hygeia.J.D.Med 2012;4(2):1-5.available from: http://www.hygeiajournal.com/Abstract.aspx?did=159.