Hygeia journal for drugs and medicines


Preliminary Phytochemical, antimicrobial and toxicity studies on Clerodendrum paniculatum Linn leaves

Hygeia.J.D.Med.4(1),April 2012-October 2012,41-50.

AuthorsPraveen.M1, Radha K*1, Hari Kumar R1, Padmaja V1, Anne Mathew1, and Ajith Kumar P2


    1. College of Pharmaceutical Sciences, Medical College, Trivandrum, Kerala, India.

2. Sree Narayana Guru Institute of Medical Sciences, Ernakulum, Kerala, India.


PlanThe present study was undertaken for investigating and scientifically validating the traditional claims of Clerodendrum paniculatum Linn   for its therapeutic activity.

MethodologyPhytochemical screening demonstrated the presence of carbohydrates, glycosides, and tannins and Phenolic compounds in methanolic and aqueous extracts and sterols in all the extracts used. The brine shrimp toxicity assay was carried out on Petroleum ether, Chloroform, Ethyl acetate, Methanol and aqueous extracts using standard procedure. Brine shrimp assay (BSA) test showed   LC50 of 400, 1600, 1700, and 1700 µg for the Petroleum ether, Chloroform, Ethyl acetate, Methanol extracts respectively. The degree of lethality was found to be directly proportional to the concentration of the extract. Maximum mortality rate of 100% was observed in the petroleum ether extract at 1600µg/ml while least mortality was observed in methanolic extract of 100 and 200µg/ml and 10% at 400 and 800µg/ml 

Outcome: In summary petroleum ether extract has the highest mortality rate than compared to methanolic extract. The methanolic and chloroform extracts of the leaves of C.paniculatum showed effective antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans.


Keywords: Clerodendrum paniculatum leaves Artemia salina, BSA method, antimicrobial activity, antifungal activity

Article history: 

Received: 2 June, 2011,

Revised: 8 August  2011,

Accepted: 05 January 2012

Available online: 05April 2012

Article outline


2.Materials and methods 

3.Results and discussion 


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