Abstract

THE EFFECTS OF Acanthospermum hispidum EXTRACT ON THE ANTI BACTERIAL ACTIVITY OF AMOXYCILLIN AND CIPROFLOXACIN

HygeiaJ. D. Med. Vol.3 (1) April 2011- September 2011, 58-63.

Type of Article:Research article

Authors:  Adu F.1*, Gbedema S.Y.1, AkanwariwiakW. G.2, Annan K.3, Boamah V.E.1

Affiliations:

1.   Department ofPharmaceutics, Faculty of Pharmacy, Kwame Nkrumah University ofScience andTechnology,  (KNUST),               

      Kumasi, Ghana.

2.   Department of Biology, Collegeof science,KNUST, Kumasi, Ghana.

3.   Department of Pharmacognosy,Faculty of Pharmacy, KNUST, Kumasi, Ghana.

Article history:

Received:1December, 2010,

Revised: 12January, 2011,

Accepted: 20February, 2011, 

Availableonline: 14 April 2011

Abstract

  The effect of the  sub-inhibitory concentrations of Acanthospermum hispidum extract on the activities of amoxicillin and ciprofloxacin was assessed by the Kirby-Bauer disc diffusion method. The extract demonstrated antibacterial activity against Staphylococcus aureus, Klebsiella pneumoniae, Bacillussubtilis,Salmonella thyphi, Proteus vulgaris and Pseudomonasaeruginosa with MICs ranging between 11 and 53 mg/ml.  A 5 mg/mlextract enhanced significantly (p<0.05) ciprofloxacin activity (up to 38 folds) against all the test bacteria except P. aeruginosa. Amoxicillin activity was also potentiated significantly (p<0.05) against Staph. aureus (9fold) and B.Subtilis (12 fold). A. hispidum appears to contain phyto constituents that may be useful adjuvant for antibiotic  formulations.

Key words: Sub-inhibitory concentration, antibacterial activity, amoxicillin,ciprofloxacin, A. hispidum. 

1. Introduction

2. Materials and methods

3. Antimicrobial  activity determination

4. Antibiotic  resistance modulation assay

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